Periodontal repair in dogs: Recombinant human bone morphogenic protein-2 significantly enhances periodontal regeneration
The purpose of this study was to evaluate bone and cementum regeneration following periodontal reconstructive surgery using recombinant human bone morphogenic protein-2 (rhBMP-2) in six beagle dogs. Surgically created mandibular supraalveolar premolar tooth defects in contralateral jaw quadrants were randomly assigned to receive rhBMP-2 or control vehicle. Clinical defects height was prepared to 5 mm; rhBMP-2 was applied with synthetic bioerodible particle and autologous blood, using 20 micrograms per 20 microliters. Flaps were advanced to submerge the teeth and sutured. The dogs were sacrificed at eight weeks postsurgery. Histometric recordings were evaluated. Histometric defect heights for rhBMP-2 and control defects were 3.7 mm and 3.9 mm respectively. The height of alveolar bone regeneration amounted to 3.5 mm and 0.8 mm for rhBMP-2 and control defects respectively. Corresponding values for bone area were 8.4 and 0.4 mm respectively. Cementum regeneration was observed in all experimental defects (17/17) and in 15 out of 17 controls, averaging 1.6 and 0.4 mm for rhBMP-2 and control respectively. Small amounts of root resorption were seen in rhBMP-2 defects, whereas controls exhibited substantial resorption. When present, ankylosis was limited and localized in both rhBMP-2 and control defects, suggesting it was unrelated to the experimental variable. The study concludes by suggesting that wound conditioning with rhBMP-2 has a significant potential for stimulating periodontal regeneration. [P.W.]
Sigurdsson, J., M. Lee, K. Kubota, T. Turek, J. Wozney, and U. Wikesjo, J Periodont, 66:125, 1995