Prostaglandin E2 regulated gingival mononuclear cell immunoglobulin production
The aim of this investigation was to determine whether PGE2 regulated immunoglobulin production within inflamed gingival tissue. The gingival mononuclear cells were harvested from inflamed gingival tissue of 20 patients with chronic adult periodontitis (AAP case types II, III, and IV). Cells were isolated and prepared for evaluation. Utilizing ELISA, elevated levels of IgG were detected in the supernatant of cultured gingival mononuclear cells. Inclusion of indomethacin, which inhibits arachidonic acid metabolites such as PGE2, caused a decrease in IgG levels. PGE2 exerted a biphasic effect upon IGG production, with high doses diminishing and low doses increasing IgG levels. From a clinical perspective, these results suggest that elevated levels of PGE2 associated with inflammation will attenuate an IgG response, and as PGE2 production wanes, the local humoral response will rebound. These findings support the theory that local levels can regulate immunoglobulin production and potentiate cytokine-induced class switching within gingival tissue. [P.W.]
Harrell, J., and S. Stein, J Periodont, 66:222, 1995