Prostaglandin E2 secretion, cell maturation, and CD14 expression of monocyte-derived macrophages from localized juvenile periodontitis patients
The purpose of this study was to compare prostaglandin E2 secretion by freshly isolated peripheral blood monocytes from localized juvenile periodontitis (LJP) and control subjects to in vitro monocyte-derived macrophages from the same subjects. An evaluation of the cell surface expression of the CD15 lipopolysaccharide (LPS) receptor in the monocyte and mature macrophage also was carried out during the culture period.
Freshly isolated monocytes (FIM) from LJP and control subjects were stimulated by LPS for 24 hours. This was performed immediately following cell separation and after 10 days in culture. By this time the FIM had differentiated into macrophages. Prostaglandin levels were measured by radioimmunoassay. CD14 also was measured by cell- ELISA. Initially, LPS from LJP subjects secreted three to four times more prostaglandin E2 (PGE2) than the control subjects. After 10 days, no measurable difference occurred between the two groups: the LJP macrophages reduced to the control macrophage level of PGE2 secretion.
This level of secretion was now equal to that seen in the FIM controls. The expression of CD14 by LJP FIM was much lower than that on the control FIM; however, after 5-10 days in culture, the macrophages from both the control and the LJP subjects expressed similar CD14 levels.
The results of this study indicate an inverse relationship between the PGE2 secretion activity and the CD14 expression of freshly isolated monocytes. The FIM of the LJP patients are high PGE2 secretors and express more CD14 than LJP FIM. Following 10 days in culture the cellular response of both groups equalized. This study supports the need for a continued evaluation of this subject. [I.S.]
Shapira, L., W.A. Saskolne, and T.E. Van Dyke, J Periodont, 67:224, 1996