Impaired bone formation in periodontal fenestration defects in dogs following application of insulin-like growth factor (II). Basic fibroblast growth factor and transforming growth factor beta 1
Effects of topically applied growth factor combination on fibroblast migration, collagen fiber formation, and bone regeneration were studied in standardized periodontal defects in four dogs. Collagen sponges impregnated with 200 nag insulin-like growth factor II, 20 nag basic fibroblast growth factor, and 6 nag transforming growth factor beta 1 were fitted to defects made through the cortical bone into root dentin, and full-thickness mucoperiosteal flaps were repositioned and sutured. Healing was observed in 3, 7,10, and 14 days after surgery. Histometric analysis showed no differences in fibroblast and collagen density between control and growth-factor defects. Bone regeneration was significantly greater in control, which received the collagen with vehicle only, than in growth-factor defects 10 and 14 days after surgery. [E.L.H.]
Selvig, K.A., U.M.E. Wikesjo, and R.D. Finkelman, J Clin Periodont, 21:381, 1994