The effect of root conditioning with minocycline HCL in removing endotoxin from the roots of periodontally involved teeth
The aim of this study is to look at minocycline's ability to remove endotoxins in periodontally diseased teeth. Researchers took 48 teeth with radiographic evidence of 50% bone loss and clinical evidence of moderate to severe periodontitis. These teeth were extracted and immersed in different concentrations of minocycline (10 mg/ml, 5 ug/ml, 50 ~g/ml) for 10 minutes, one day, three days, and seven days. Control specimens were subjected to immersion in pirogue-free water, agitation, and polishing of citric acid for three minutes. They collected solutions and were assayed for endotoxins. The endotoxins remaining on the root were qualified by collecting cement shavings. The results showed that the mean level of total amount of endotoxin was the largest, 14.56 x103 ng/root, when treated with 10 mg/ml minocycline solution, and smallest, 4.48 x 103 ng/root, with citric acid treatment. The rates of removal of endotoxin achieved by treatment with a 10 mg/ml minocycline solution for 10 minutes, one day, or three days was the highest, being statistically more significant than the 5 ug/ml and 50 ~g/ml group. The group treated with the pyrogen-free water for seven days had a rate of 95.8%, showing no difference from the minocycline groups.
The group treated with polishing or citric acid showed a higher rate than the group treated with 10 mg/ml of minocycline. In the present study, the removal rate of endotoxin with a 10 mg/ml minocycline solution for 10 minutes was the highest, 50%. The authors suggest combining polishing with a chemical root preparation method and conventional root planing. [M.C.R.]
Minabe, M., K.Takeuchi, H. Kumada, and T. Umemoto, J Periodont, 65:387, 1994