Cell Suspension Comparisons of Bone Production in Bone Marrow Stem Cells of Young and Adult Aged Rats as Measured In Vitro by Bone Colony Assays

Stephanie A. Dodson

Member's Homepage | PIC Homepage
TABLE OF CONTENTS
  1. Abstract
  2. Introduction
  3. Chapter One
  4. Chapter Two
  5. Chapter Three
  6. Chapter Four
  7. References

CHAPTER THREE

RESULTS OF COMBINED TRIALS

The results for the combination of red marrow only and red marrow with the addition of fatty marrow are presented in the form of tables. In addition the number of stem cells per animal and the number of stem cells per million cells plated are reported.

TABLE 1.
Total Number of Osteogenic Colonies

Red Marrow Only:
Aged: 0.045 +/- 0.6863
Young: *3.6 +/- 2.3523

There was a statistically significant increase in the number of osteogenic colonies derived from the young adult animals as compared to the aged animals for red marrow only * P < 0.000 Student's t Test.

Red Marrow with Fatty Marrow Added:
Aged: 1.125 +/- 1.1260
Young: ~3.8333 +/- 1.4720

When the fatty marrow was added to the red marrow, there was no statistically significant increase in the number of osteogenic colonies over that of red marrow only. However, there was a statistically significant increase in the number of colonies derived from young adults as compared to aged animals. ~ P< 0.002 Student's t Test.

TABLE 2.
Total Size of Osteogenic Colonies

Red Marrow Only:
Aged: 0.1250 +/- 0.1033
Young: 0.1276 +/- 0.0518

Fatty Marrow Added:
Aged: 0.08803 +/- 0.06094
Young: 0.1285 +/- 0.0285

There was no Statistically significant difference in the size of osteogenic colonies between aged and young adults, either with red marrow only, or the addition of fatty marrow.

TABLE 3.
Total Number of Hemopoietic Colonies

Red Marrow Only:
Aged: 5.25 +/- 2.2449
Young: ~8.23 +/- 3.3601

There was a statistically significant increase in the number of hemopoietic colonies derived form young adults as opposed to aged animals with red marrow only. Student's t Test ~ P < 0.002.

Red Marrow With Fatty Marrow Added:
Aged: ~, *19.62 +/- 6.7387
Young: 6.5 +/- 1.8708

There was a statistically significant difference in the number of hemopoietic colonies derived from aged animals over young animals, Student's t Test P < 0.002 and there was a statistically significant increase in the number of hemopoietic colonies derived from aged animals with red marrow only and the addition of fatty marrow P < 0.000. There was no statistically significant difference in the number of hemopoietic colonies derived from young adults with red marrow only or red marrow with fatty marrow added.

TABLE 4.
Total Size for Hemopoietic Colonies Reported in Square Millimeters

Red Marrow Only:
Aged: 0.07591 +/- 0.0514
Young: 0.06010 +/- 0.0172

Red Marrow With Fatty Marrow Added:
Aged: 0.0506 +/- 0.0126
Young: 0.0485 +/- 0.0185

There was no statistically significant difference noted for the sizes of hemopoietic colonies for any group.

TABLE 5.
The Number Of Light Density Cells Harvested From Aged And Young Animals
I. Red Marrow Only:
Age # Animals # Light Density Cells # Cells/Animal
Young 6 50 x 106 8.3 x 106
Young 5 47 x 106 9.4 x 106
Young 5 47 x 106 9.4 x 106
Young 4 54 x 106 13.5 x 106
Young 4 86 x 106 21.6 x 106

x = ~13.2000 +/- 6.0305

II. Red Marrow Only:

Age # Animals # Light Density Cells # Cells/Animal
Aged 4 33 x 106 8.25 x 106
Aged 4 25.6 x 106 6.4 x 106
Aged 4 13.4 x 106 3.35 x 106
Aged 4 4.25 x 106 2.125 x 106
x = 5.0312 +/- 2.7992
Statistically Significant:
Students t Test: ~ P < 0.05

III. Red Marrow with Fatty Marrow Added

Age # Animals # Light Density Cells # Cells/Animal
Young 6 75 x 106 12.5 x 106

IV. Red Marrow with Fatty Marrow Added

Age # Animals # Light Density Cells # Cells/Animal
Aged 3 70 x 106 *23.3 x 106

Student's t Test: * P < 0.001

There was a statistically significant increase in the number of light cells harvested from the young adults over the aged animals when comparing red marrow only Student's t Test P < 0.05.

When the fatty marrow was added, there was no statistically significant difference in the number of light cells harvested from young animals with red marrow only. However, there were statistically significant differences in the number of light density cells harvested from aged animals over red marrow only Student's t Test P < 0.001.

TABLE 6.
The Number of Stem Cells per One Million Cells Plated

Red Marrow Only:
Osteogenic:
Aged: 0.015
Young: *1.2

Hemopoietic:
Aged: 1.75
Young: ~2.73

There was a statistically significant increase in the number of stem cells per million cells plated between aged and young animals both with red marrow and fatty marrow added. Studentıs t Test * P < 0.000 and ~ P < 0.002.

Red Marrow With Fatty Marrow Added:

Osteogenic:
Aged: 0.41
Young: ~1.26

Hemopoietic:
Aged: *,~ 6.5
Young: 2.16

Top of Page